Easyselect tm pichia expression kit
WebJun 30, 2024 · The recombinant plasmid was isolated, linearised and transformed into competent Pichia pastoris X33 cells using electroporation. The Pichia transformants were determined using PCR and expressed... WebYeast expression technology is widely available and used to produce, for example, the VLP antigen of the licensed HPV vaccine as well as recombinant hepatitis B vaccines in several middle-income countries (LMICs),22 including Bangladesh, Brazil, Cuba, India, and Indonesia, over the last 35 years.
Easyselect tm pichia expression kit
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WebThe original pichia expression kit provides all of the tools and reagents needed to express a gene of interest from the aox1. Web the easyselect pichia expression kit uses the … WebOur new pichiapink yeast expression system contains both low and high copy plasmid backbones, 8 secretion. Web for the original pichia expression kit and the …
WebFeb 20, 2016 · P. pastoris GS115 expressing laccase (GSLAC) was constructed according to the easy select Invitrogen protocol (EasySelect TM Pichia expression kit) and used in this study for comparison with the empty P. pastoris GS115 strain. Copper sulphate pentahydrate (CuSO 4 ·5H 2 O) was used throughout the study as a copper source. All … Webvii Kit Contents and Storage, Continued Shipping and Storage The EasySelect™ Echo™-adapted Pichia Expression Kit is shipped on dry ice except for the EasySelect™Pichia …
Webin the manual for EasySelectTM Pichia Expression Kit of Invitrogen. The transformants were screened on YPDS ? ZeocinTM plates (1% yeast extract, 2% peptone, 2% dextrose, 1 M sorbitol, 2% agar, 100 lg/ml ZeocinTM) and the ZeocinTM-resistant Pichia colonies were replica-plated onto MDH and MMH plates (1.34% yeast nitrogen WebOct 12, 2014 · According to the EasySelect Pichia Expression Kit protocol, a freshly prepared 80 μL aliquot of electrocompetent P. pastoris KM71H strain and 5 μg recombinant expression vector DNA linearized with SstI was used for transformations according to the manufacturer’s instructions using the Bio-Rad Gene Pulser and Pulse Controller at pulse ...
Webaccording to the Pichia expression system manual from Invitrogen and were used to maintained clones which were stored at 4oC. All media were prepared according to the Invitrogen manuals (EasySelectTM Pichia Expression Kit user guide Cat. no. K1710-01 and P. pastoris Fermentation Process Guidelines, 2010) and
WebMany of the reagents supplied in the EasySelect™ Pichia Expression Kit are available separately from Invitrogen. Ordering information is provided below. Product Reactions or … euroleague women\\u0027s basketball 2023WebEasySelect Pichia Expression Kitには、プラスミドpPICZ A、B、C、およびpPICZα A、B、Cそれぞれ20 µg、Pichia pastoris株X-33（野生型）、GS115（his4）、KM71H（arg4 aox1:: ARG4）、細胞内発現と分泌発現用のコントロール株、Zeocin™抗生物質250 mg、培地（YPおよびYNB）、EasyComp™ Pichia Transformation Kit、および、5´ AOX1 … first affiliated collingwoodWebAug 1, 2011 · The expression of recombinant plectasin in P. pastoris was performed according to the EasySelect TM Pichia Expression Kit (Invitrogen, Beijing, China). Positive transformants were cultured at 30 °C in a shaking flask containing 10 ml of BMGY medium until reaching an OD 600nm of 4.0. first aer lingus flightWebKit contents The PichiaExpression Kitis shipped at room temperature and contains the following components. Spheroplast Module (Box 1). Store at room temperature. Reagent … eurolearn indiaeuro leasing berlinWebThe Original Pichia Expression Kit provides all of the tools and reagents needed to express a gene of interest from the AOX1 promoter. For Research Use Only. Not for use in diagnostic procedures. Specifications Antibiotic Resistance Bacterial Ampicillin (AmpR) Bacterial or Yeast Strain GS115, KM71 Cloning Method Restriction Enzyme⁄ MCS first aestheticsWebAug 14, 2024 · All vectors were from Thermo Fischer Scientific. Constructs were verified by sequencing. The fungal PGs (including the His 6 and V5 epitope) were expressed in the yeast P. pastoris according to the manufacturer's instructions of the EasySelect TM Pichia Expression Kit (Thermo Fischer Scientific). first affiliated hospital if anhui university