WebSep 16, 2014 · Finally, 1×10 7 vital cells were used for isolation of GD 2 -positive cell subset with MACS technique (Miltenyi Biotec, Teterow, Germany). For this purpose 1×10 7 primary cells were resuspended in 200 µl 1x PBS/0.5% BSA/2 mM EDTA buffer (pH 7.4) and then incubated on ice with 20 µl FcR-blocking reagent (Miltenyi Biotec, Teterow, … Webtissue, The Tumor Dissociation Kit (Mitenyi Biotec, Teterow, Germany) was used. RNA isolation and quantitative PCR Total RNA was extracted from 1.0×10 5 cells, and cDNA was synthesized with AMV-reverse transcriptase and random hexamer primers (Roche Diagnostic, Indianapolis, Indiana, USA). Quantitative PCR (qPCR) was performed

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WebFor Mydgf recombinant protein experiments, CMs were isolated from hearts of WT mice at P1 using the Neonatal Heart Dissociation Kit in combination with gentleMACS TM and Octo Dissociator (Miltenyi BioTec, Teterow, Germany) according to … WebMiltenyi Biotec Salaries trends. 8 salaries for 6 jobs at Miltenyi Biotec in Teterow. Salaries posted anonymously by Miltenyi Biotec employees in Teterow. green witch oracle cards by cheralyn darcey

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